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Biji T. Kurien; R. Hal Scofield
Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In ?Protein Blotting and Detection: Methods and Protocols?, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology? series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
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The next time you're feeling a little blue, try singing a song to lift your spirits. That's something that I've often done (without knowing the science behind it), but we're curious, Lifehackers: what do you do to quickly boost your mood? 
Meg Whitman 
The New York Police Department put American citizens under surveillance and scrutinized where they ate, prayed and worked, not because of charges of wrongdoing but because of their ethnicity, according to interviews and documents obtained by The Associated Press.
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